02 · UV-Vis
Quantify, characterise and follow reactions in solution
A double-beam UV-Visible spectrophotometer measuring absorbance and transmittance across the ultraviolet and visible region — for concentration determination, electronic-transition studies, kinetics and band-gap estimation.
- Optics
- Double-beam
- Wavelength range
- 190 – 1100 nm (typical)
Working principle
Light from deuterium (UV) and tungsten-halogen (visible) lamps is dispersed by a monochromator, and a narrow band of wavelengths passes alternately through the sample and a reference cuvette. The detector compares the two beams to give absorbance at each wavelength.
Molecules absorb UV and visible light when electrons are promoted to higher energy levels — π→π*, n→π* and charge-transfer transitions — so the position and intensity of absorption bands reveal conjugation, metal–ligand interactions and nanoparticle plasmon resonance.
The Beer–Lambert law (A = εcl) makes absorbance directly proportional to concentration. Using a calibration curve of standards, the instrument determines unknown concentrations with high precision.
Representative spectrum · for illustration
Technical specifications
- Optics
- Double-beam
- Wavelength range
- 190 – 1100 nm (typical)
- Measurement modes
- Absorbance, % Transmittance, concentration, kinetics
- Cuvettes
- 10 mm quartz (UV) and glass cells
- Data formats
- PDF report, CSV / ASCII
Typical turnaround: 2–3 working days after sample receipt
Capabilities
- Absorbance & transmittance measurement
- Concentration determination (Beer–Lambert calibration)
- Full-range wavelength scans and λmax
- Reaction kinetics / time-course studies
- Optical band-gap estimation (Tauc plot) of solutions and dispersions
- Nanoparticle surface plasmon resonance
Samples accepted
- Clear solutions
- Dilute colloidal dispersions
- Transparent liquids
Sample requirements
- Provide at least 3–5 mL of solution per sample
- Mention the solvent — it is used as the reference blank
- Absorbance is most reliable between 0.1 and 1.5; dilute concentrated samples
- Solutions should be clear and free of suspended particles
- For concentration work, bring standards or specify the calibration range
Applications
Analytical chemistry
Quantitative estimation of drugs, dyes, metal ions and biomolecules using calibration curves.
Nanomaterials
Confirm synthesis of silver and gold nanoparticles through their plasmon bands; follow growth and stability.
Photocatalysis & environment
Track dye degradation over time and estimate rate constants.
Biochemistry & life sciences
Protein and nucleic acid estimation, enzyme assays and antioxidant studies.
Service charges
Available on request
Charges depend on the technique, the number of samples and your user category (internal, academic or industry). Submit an enquiry and we will email you a quotation.
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