03 · PL
Ultra-sensitive emission studies down to trace levels
Records excitation and emission spectra of fluorescent molecules and materials — for sensing, quantum-dot and dye studies, quenching experiments and photophysical characterisation.
- Light source
- Xenon lamp
- Modes
- Emission, excitation and synchronous scans; time scans
Working principle
A xenon lamp and an excitation monochromator select a wavelength that the sample absorbs. Excited molecules relax and emit light at longer wavelengths; this emission is collected at right angles to the excitation beam, passed through a second monochromator and measured by a sensitive photomultiplier detector.
Because emission is measured against a dark background, fluorescence is often a thousand times more sensitive than absorbance. Changes in intensity, wavelength or shape of the emission reveal binding events, the local environment, and energy or electron transfer.
Representative spectrum · for illustration
Technical specifications
- Light source
- Xenon lamp
- Modes
- Emission, excitation and synchronous scans; time scans
- Wavelength range
- Approx. 200 – 900 nm (typical)
- Geometry
- 90° detection, 10 mm quartz cuvette
- Data formats
- PDF report, CSV / ASCII
Typical turnaround: 3–5 working days after sample receipt
Capabilities
- Emission and excitation spectra
- Fluorescence quenching (Stern–Volmer) studies
- Sensing and detection-limit studies
- Relative quantum-yield estimation
- Synchronous fluorescence
Samples accepted
- Dilute solutions
- Colloidal dispersions
- Quantum / carbon dot suspensions
Sample requirements
- Provide 3–5 mL of a dilute solution (absorbance below 0.1 at the excitation wavelength)
- Mention the solvent and the expected excitation / emission region
- Use spectroscopic-grade solvents where possible
- Protect light-sensitive samples from light during transport
Applications
Chemical sensors
“Turn-on / turn-off” probes for metal ions, anions and explosives; detection-limit calculation.
Quantum dots & carbon dots
Emission tuning, excitation dependence and photostability of nanomaterials.
Bio-interactions
Binding of drugs and probes with proteins and DNA through quenching studies.
Photochemistry
Energy and electron-transfer studies in dyes, complexes and photocatalysts.
Service charges
Available on request
Charges depend on the technique, the number of samples and your user category (internal, academic or industry). Submit an enquiry and we will email you a quotation.
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Track every step online — from review and quotation to analysis and report.